Goat Antiserum to Horse Serum, 2mL

The total protein is measured using the Biuret procedure, with bovine albumin as the standard; The product has been tested at 60 mg/mL and found negative against the following whole sera at 20-25 mg/mL: Bear, Deer, Hamster, Rabbit, Turkey, Bovine, Dog, Human, Rat, Cat, Goat, Monkey, Sheep, Chicken, Guinea, Pig, Mouse, Swine. The antiserum is prepared by immunizing goats with horse serum; antiserum is delipidated and made specific by immunoadsorption with soluble antigens or solid-phase immunoadsorption where appropriate. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. Whole serum polyclonal antibody is used for immunostaining of cell suspensions, acetone-fixed frozen tissue sections, and formalin-fixed, paraffin-embedded tissue sections. This product is intended for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis, capillary tube, and immunodiffusion techniques. Product is the lyophilized powder of goat antiserum to horse serum and buffer salts. Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. If a small amount of precipitate is present, or if preparation appears cloudy, clarify by centrifugation or filtration.