GOAT IGG ISOTYPE CONTROL COLORWHEEL DYE-READY PAB

General description

ColorWheelColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheelColorWheel^® antibody with any ColorWheelColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheelColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheelColorWheel^® technology requires both ColorWheelColorWheel^® antibody and ColorWheelColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

This is an isotype control polyclonal antibody for Goat IgG.

Immunogen

Unknown

Application

Quality Control Testing

Evaluated by Flow Cytometry with Protein A/G bead.

Flow Cytometry Analysis: Goat IgG Isotype Control ColorWheelColorWheel^® Dye-Ready pAb was tested by flow cytometry using Protein A/G beads.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Goat IgG Isotype Control is suitable for use an isotype-matched control for primary antibodies made in goat. It has unknown binding specificity and can be used for evaluation of background staining caused by non-specific interactions between Goat IgG antibody and the sample under defined experimental conditions. It is suitable for use in multiple applications.

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.