Golgi-Tracker Green

Precautions：For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.Coverslips and slides are required but not supplied, They can be ordered from .Golgi-Tracker Green is suitable for staining Golgi in live cells, but not Golgi in fixed cells.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1.Preparation of Golgi-Tracker Green working solution a.Dilute an appropriate amount of Golgi-Tracker Green (33.3mg/ml) in the Golgi-Tracker Red Dilution Buffer at a ratio of 1:100. For example, mix 1µl of Golgi-Tracker Green with 1ml of Golgi-Tracker Green Dilution Buffer to obtain the Golgi-Tracker Green working solution.Note: The dilution ratio of Golgi-Tracker Green can be adjusted within the range of 1:50-1:200 based on the staining results.b.Golgi-Tracker Green working solution can be recovered and stored at 4℃ or -20℃ for future uses until it no longer works. Store at 4℃ if it will be reused within 1-2 days , or at -20℃ for longer period of storage.2.Fluorescent labeling of Golgi bodies in live cellsa.Remove the cell culture medium and wash the cells grown on coverslips with an appropriate amount of solution such as Hanks' Balanced Salt Solution with Ca2+ & Mg2+ (, C0219). For staining of suspension cells, centrifuge to collect cells and refer to the staining method for adherent cells.b.Remove the wash buffer, add the Golgi-Tracker Green staining working solution prepared in step 1 and incubate at 4℃ for 30 min.c.Recover the Golgi-Tracker Green staining working solution, wash the cells about 3 times with cell culture medium pre-cooled on ice, and incubate with fresh culture medium at 37℃ for 30 minutes.d.Wash again with fresh culture medium, followed by observation under fluorescence microscope or confocal microscope. Bright green fluorescent Golgi bodies can be observed, while other membrane systems within cells show a fainter fluorescent staining.Related Products：Cat. No.Product NamePack Size C1002 DAPI 5mg/ml×0.2ml C1005/C1006 DAPI staining solution 10ml/50ml C1011 Hoechst 33258 10mg C1017/C1018 Hoechst 33258 Staining Solution 10ml/50ml C1022 Hoechst 33342 10mg C1025/C1026 Hoechst 33258 Staining Solution 10ml/50ml C1027/C1028/C1029 Hoechst 33342 Staining Solution for Live Cells (100X) 0.1ml/0.5ml/3ml C1033 Actin-Tracker Green (microfilament green fluorescent probe) 0.2ml C1036 DiI (cell membrane red fluorescent probe) 10mg C1038 DiO (cell membrane green fluorescent probe) 10mg C1039-10mg DiD (cell membrane far-infrared fluorescent probe) 10mg C1041 ER-Tracker Red (Endoplasmic Reticulum Red Fluorescent Probe) 20μl C1042S ER-Tracker Green (Endoplasmic Reticulum Green Fluorescent Probe) 20μl C1043 Golgi-Tracker Red (Golgi red fluorescent probe) 1mg C1045S Golgi-Tracker Green (Golgi green fluorescent probe) 1mg C1046 Lyso-Tracker Red (Lysosomal Red Fluorescent Probe) 50μl C1047S Lyso-Tracker Green (lysosomal green fluorescent probe) 50μl C1048 Mito-Tracker Green (mitochondrial green fluorescent probe) 50μg C1049-50µg Mito-Tracker Red CMXRos (Mitochondrial Red Fluorescent Probes) 50μg C1049-250µg Mito-Tracker Red CMXRos (Mitochondrial Red Fluorescent Probes) 50μg×5 C1050 Tubulin-Tracker Red (antibody-based microtubule red fluorescent probe) 40μl C1051S Tubulin-Tracker Green (antibody-based microtubule green fluorescent probe) 40μl C1991S Red fluorescence staining kit for cell membrane (DiI) 200-1000T C1993S Green fluorescent staining kit for cell membranes (DiO) 200-1000T C1995S Far-infrared fluorescence staining kit for cell membrane (DiD) 200-1000T C2005 JC-1 1mg C2007 Rhodamine 123 5mg