GRAM'S CRYSTAL VIOLET SOLUTION* (C005B-238185)

General description

Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.

Application

• Crystal violet is routinely used for Gram staining and its variants, staining amyloid, bacterial components, and vascular plant tissues.
• It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyze meiotic structures.
• It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears.

Biochem/physiol Actions

The antimicrobial effects of crystal violet are caused by free radical generation and the formation of unionized dye-bacteria complexes. It also inhibits bacterial protein synthesis and cell wall formation and is especially effective against Gram-positive bacteria.

Principle

Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms complexes with Gram’s iodine that are entrapped and retain the purple color even after decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner and does not retain crystal violet.

grade: for microscopy. Quality Level: 200. product line: BioChemika. form: liquid. shelf life: limited shelf life, expiry date on the label. technique(s): microbe id | staining: suitable. density: 0.981 . g/mL . at 20 . °C. ε. _max: 33.8 at 585-595 . nm in water. antibiotic activity spectrum: Gram-negative bacteria, Gram-positive bacteria. application(s): diagnostic assay manufacturing
hematology
histology. storage temp.: room temp. Pictograms: GHS02,GHS07,GHS08. Signal Word: Warning. Hazard Statements: H226 - H319 - H351 - H412. Precautionary Statements: P202 - P210 - P233 - P273 - P305 + P351 + P338 - P308 + P313. Hazard Classifications: Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 3. Storage Class Code: 3 - Flammable liquids. WGK: WGK 2. Flash Point(F): 93.2 °F. Flash Point(C): 34 °C. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter.

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