Solution IA - Crystal Violet
Solution IB - Sodium Bicarbonate, 5% (wt/vol)
Solution II - Iodine
Solution III - Acetone Alcohol
Solution IV - Safranin

The Kopeloff's Modification of the traditional Gram Stain technique.

CONTENTS: Crystal Violet, Ethanol, Potassium Iodide, Iodine, Acetone, Safranin, Basic Fuchsin, Phenol Crystal Violet, Sodium Bicarbonate, Sodium Hydroxide, Potassium Iodide, Iodine, Ethanol, Acetone, Safranin

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE FOR OPTIMUM RESULTS: 1. Cover the fixed slide with Solution IA and then add 5 drops of Sodium Bicarbonate and blow on slide to mix. Let stand for 15 seconds. Continue staining as usual for Gram Stain. Wash off gently with distilled water.

NOTE: Crystal Violet is used because it is more stable than Gentian Violet. 2. Apply Solution II, (Kopeloff's Iodine modification) for 1 minute and wash off gently with distilled water. 3. Decolorize with Solution III, Acetone Alcohol until the purple color no longer runs off the slide. (From 2 to 20 seconds depending upon thickness of specimen) Wash with distilled water. 4. Cover slide with Solution IV, Safranin for 1 minute. Wash with distilled water and allow to dry.

RESULTS: Gram-positive bacteria stain purple to blue-black. Gram-negative bacterial stain pink to red.

Safety Data Sheet - Gram Stain Kit - Kopeloff's Modification with Safranin (Solution IA)

Safety Data Sheet - Gram Stain Kit - Kopeloff's Modification with Safranin (Solution IB)

Safety Data Sheet - Gram Stain Kit - Kopeloff's Modification with Safranin (Solution II)

Safety Data Sheet - Gram Stain Kit - Kopeloff's Modification with Safranin (Solution III)

Safety Data Sheet - Gram Stain Kit - Kopeloff's Modification with Safranin (Solution IVA)