Granulocyte Macrophage Colony Stimulating Factor; Rhesus Mac (C08-0302-981)

G-CSF, Granulocyte Macrophage Colony Stimulating Factor, monkey (rhesus macaque): Rhesus macaque Granulocyte Colony Stimulating Factor GCSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNFα and IFNγ. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete GCSF after LPS, IL1 or TNFα activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express GCSF constitutively. Source: Escherichia coli. Molecular Weight: Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. Quantity: 2ug/10ug/1mg AASequence: TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEEL VLLRHSLGIP WAPLSSCPSQALQLTGCLSQLHSSLFLYQGLLQALEGISPELSPTLDT LQLDIADFAT TIWQQMEDLGMAPALQPTQGAMPAFTSAFQRRAGGVLVASHLQRF LELAYRVLR HLAQS Purity: >98% by SDS-PAGE and HPLC analyses. Biological Activity: The ED50 as calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 1.0×107 IU/mg. Formulation: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4. Endotoxin: Less than 1EU/mg of rRhG-CSF as determined by LAL method. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions