Granzyme B Substrate I, Colorimetric - Calbiochem (C15-1303-059)

General description

Colorimetric substrate for detection of granzyme B. Cleavage is monitored colorimetrically at ~405 nm.

Colorimetric substrate for the detection of granzyme B and caspase-8 activity. Cleavage of pNA is monitored colorimetrically at ~405 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Colorimetric substrate for the detection of granzyme B and caspase-8 activity

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Ile-Glu-Thr-Asp-pNA

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Koeplinger, K.A., et al. 2000. Protein Exp. Purif. 18, 378.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 638.62. Empirical Formula: C₂₇H₃₈N₆O₁₂. Quality Level: 100. Assay: ≥. 95% (HPLC). form: lyophilized solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, protect from light. color: white. solubility: DMSO: 5 . mg/mL. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.