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Griess reagent (modified)

Sigma-Aldrich

Catalog No.
C15-1425-665
Manufacturer No.
G4410-10G
Manufacturer Name
Sigma-Aldrich
Quantity
10
Unit of Measure
GR
Price: $279.77
List Price: $310.86

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid.

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General description

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.

Application

Griess reagent (modified) has been used:

  • in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
  • for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
  • for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells
  • to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)
  • to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants

UPC:
12352103
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
G4410-10G


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