Cell Line Origin
Human Caucasian skin fibroblast Gardner′s syndrome
Cell Line Description
Derived from a 13 year old Caucasian male with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. The modal chromosome number was 46, occurring in 60% of cells.
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,12
D16S539: 12,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,8
TPOX: 8,11
vWA: 14,17
Culture Medium
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Monolayer should form in a 25cm2 flask in 3-4 days.
Other Notes
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biological source: human skin. growth mode: Adherent. karyotype: Modal no. 46. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41105328
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90110503-1VL
