GSK-3beta, His.Tag, Human, Recombinant, E. coli (C15-1303-038)

General description

Recombinant, human GSK-3β fused at the N-terminus to a His•Tag sequence and expressed in E. coli. A dual specificity kinase that plays important roles in insulin- and Wnt-mediated cellular signalings. In AD (Alzheimer’s disease), GSK3β has a major role in microtubule stability by its ability to phosphorylate the microtubule associated protein tau. Its known substrates include glycogen synthase, p90^rsk, Tau, c-Jun, and CREB. MW: 53,000-56,000.

Recombinant, human GSK-3β fused at the N-terminus to a His•Tag sequence and expressed in E. coli. Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. GSK-3β is a dual-specificity kinase that is important role in insulin- and Wnt-mediated cellular signaling events. In Alzheimer′s disease GSK-3β is reported to affect microtubule stability via the phosphorylation of microtubule associated protein, Tau. Other known substrates include glycogen synthase, p90^rsk, c-Jun, and CREB. MW: 53,000-56,000

Biochem/physiol Actions

1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.1 M NH₄CO₃.

Reconstitution

Following reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.

Reconstitute in sterile PBS to a final concentration of 1 mg/ml.

Other Notes

Harwood, A., and Braga, V.M. 2003. Nat. Cell Biol.5, 275.
Bhat, R.V., and Budd, S.L. 2002. Neurosignals11, 251.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

recombinant: expressed in E. coli. Quality Level: 100. Assay: ≥. 90% (SDS-PAGE). form: solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.