InformationGSK J4 HCl (GSKJ4 HCl) GSK J4 HCl is a cell permeable prodrug of GSK J1, which is the first selective inhibitor of the H3K27 histone demethylase JMJD3 and UTX with IC50 of 60 nM in a cell-free assay and inactive against a panel of demethylases of the JMIn vitroGSK J4 HCl is an ethyl ester derivative of the JMJD3 selective histone demethylase inhibitor GSK-J1 with an IC50 value greater than 50 μM in vitro. GSK J4 HCl is used to probe the consequences of demethylation of H3K27me3. In human primary macrophages, GSK-J4 inhibits the lipopolysaccharide-induced production of cytokines, including pro-inflammatory tumour necrosis factor (TNF). In addition, GSK-J4 prevents the lipopolysaccharide-induced loss of H3K27me3 associated with the TNF transcription start sites and blocked the recruitment of RNA polymerase II.In vivoCell Datacell lines：MDA-468, PC-3, HCT-116 and MiaPaca cellsConcentrations：Incubation Time：Powder Purity:≥98%. Specification: 10mM in DMSO Molecular Formula: C24H27N5O2·HCl Molecular Weight: 453.96
- UPC:
- 51112518
- Condition:
- New
- Availability:
- 2 weeks
- Weight:
- 0.85 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- G408916-1ml
- CAS:
- 1797983-09-5
- Product Size:
- 1ml
