Guanidine hydrochloride (GdnHCl) is a well-known protein and enzyme denaturant. It is a small molecule and hydroscopic in nature.Application:Guanidine hydrochloride (GdnHCl) has been used as a component of the phosphate buffer saline (PBS) for the incubation of Schlemm′s canal (SC) cells. It has also been used as a component of the digestion buffer for the gelation and expansion of human HeLa229 cells. GdnHCl is also used to isolate RNA.Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Specification: BioUltra, for molecular biology, ≥99.5%(AT)
MDL Number: MFCD00013026
Molecular Formula: CH5N3·HCl
Molecular Weight: 95.53
EC Number: 200-002-3
PubChem CID: 5742
Isomeric SMILES: C(=N)(N)N.Cl
Beilstein Registry Number: 3591990
- UPC:
- 41105500
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 0.51 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- G755683-50g
- CAS:
- 50-01-1
- Product Size:
- 50g
- Hazard Statement Codes:
- H302:H319:H315
