Guanidine hydrochloride (C15-1244-126)

General description

Guanidine hydrochloride, or aminoformamidine hydrochloride, is a versatile chemical compound widely used in cell biology and biochemical research due to its potent chaotropic properties. Its ability to disrupt protein and nucleic acid structures makes it valuable for purifying proteins and nucleic acids, particularly mRNA. Guanidine hydrochloride unfolds proteins, facilitating their purification and refolding, and it is effective in solubilizing denatured insoluble proteins at higher concentrations.

At lower concentrations, guanidine hydrochloride has the intriguing ability to promote the refolding of denatured proteins, aiding in protein renaturation studies. In RNA extraction, it acts as a strong denaturant, disrupting cell structures and ensuring the integrity of extracted RNA by inactivating RNA enzymes. Overall, guanidine hydrochloride′s denaturing and renaturing properties make it an essential reagent for various cell biology applications, including protein purification, nucleic acid isolation, and protein refolding studies.

Application

Guanidine hydrochloride has been used:

• for lysing homogenized brain tissue
• in the preparation of incubation buffer for Ni-sepharose protein binding, purification, propionylation, and on-bead digestion to minimize nonspecific binding to the affinity resin
• in the preparation of solutions/extraction buffer to extract soluble protein from the human tissue

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride, an efficient chaotropic reagent, is involved in RNA isolation, globular protein denaturation, and protein refolding studies. This denaturant is appropriate for performing rapid and single-step affinity-purification mass spectrometry (AP-MS) experiments.

Features and Benefits

Highly versatile surfactant for your cell biology and biochemical research