Application
Guanidine thiocyanate has been used:
- as a denaturing agent to study its effects on surface activity of globular protein
- for the extraction of total RNA from primary hepatocytes
- as a component of the incubating buffer for extraction of DNA from human bone
- as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
- as a component of lysis solution for extraction of RNA from rat pancreatic tissues
Chaotropic agent and strong denaturant; solubilizes cells.
Biochem/physiol Actions
Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.
- UPC:
- 51472800
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- G6639-100G