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Guanidine thiocyanate (C15-1250-910)

Catalog No.
C15-1250-910
Manufacturer No.
G6639-500G
Manufacturer Name
Sigma-Aldrich
Quantity
500
Unit of Measure
GR
Price: $527.25
List Price: $585.83

Application Guanidine thiocyanate has been used: as a denaturing agent to study its effects on surface activity of globular protein for the extraction of total RNA from primary hepatocytes as a component of the incubating buffer for extraction of

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Application

Guanidine thiocyanate has been used:

  • as a denaturing agent to study its effects on surface activity of globular protein
  • for the extraction of total RNA from primary hepatocytes
  • as a component of the incubating buffer for extraction of DNA from human bone
  • as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
  • as a component of lysis solution for extraction of RNA from rat pancreatic tissues

Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.

UPC:
51472800
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
G6639-500G


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