General description
Guanidine thiocyanate is a chaotropic agent that functions as a strong protein denaturant. It is widely used in RNA isolation protocols. Guanidine thiocyanate can irreversibly inactivate RNase and DNase and breaks cellular membranes. It dissolves proteins by disturbing their secondary structures. Guanidine thiocyanate also separates nucleoproteins from nucleic acids and aids the digestion of the proteins with proteases. It improves the activity of proteinase K thus making it the protease of choice.
Application
Guanidine thiocyanate solution has been used to extract:
- plasma RNA from human immunodeficiency virus (HIV)-infected and control patients
- total RNA from collected tissues (shoots, flowers, fruits, and seeds)
- in reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA from teratocarcinoma cell line (Tera-1)
Caution
possibly crystallized product can be dissolved again by slightly heating to 40-50 °C.
- UPC:
- 51472800
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 50983-50ML
