Cell Line Origin
Mouse P3X63Ag8.653 x Rat LOU/M spleen
Cell Line Description
The antibody is directed against the murine transferrin receptor.
Immunogen
The cytolytic T cell clone A15.1.17
Application
The isotype of the monoclonal antibody produced by this cell line is rat IgG2b.
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS). Immediately post resuscitation of the cells use media containing 20% FBS, once the culture is established the level of FBS can then be reduced to 10% FBS.
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: Mouse x Rat Heterohybridoma. ImmunoDon: LOU/M rat spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-Transferrin Receptor, mouse. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51141509
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85103103-1VL