Cell Line Origin
Mouse P3X63Ag8.653 x Rat LOU/WS1 spleen
Cell Line Description
The antibody recognises a monomorphic determinant on the L3T4 molecule (the murine equivalent of CD4).
Immunogen
Lyt-1+2- anti-I-Ak cytolytic T lymphocytes
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS). Immediately post resuscitation of the cells use media containing 20% FBS, once the culture is established the level of FBS can then be reduced to 10% FBS.
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
biological source: Mouse x Rat Heterohybridoma. ImmunoDon: LOU/WS1 rat spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-L3T4, mouse. technique(s): cell culture | mammalian: suitable. isotype: IgG2a, kappa. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 12352207
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 85103101-1VL
- Temperature Control Device:
- Yes
