H2S FLUORESCENT PROBE P3 1PC X 5MG (C15-1305-151)

General description

A cell-permeable, non-toxic, non-fluorescent naphthalenyl-dimethoxybenzaldehyde enone compound that acts as a highly specific, sensitive, fast-responsive H₂S fluoroprobe (signal saturation at ≤ 5 min with a detection limit of 50 nM). Dose-dependently detects hydrogen sulfide levels under physiological conditions and readily fluoresces. Displays little to no reactivity towards GSH, Cys, H₂O₂, citrate and iodide (Hepes pH 7.4, containing 10% FBS). Also shown to be useful as a two-photon probe for imaging endogeneous H₂S levels in HeLa cells. A thiol specific fluorescent probe is also available (Cat. No. 595504).

A cell-permeable, non-toxic, non-fluorescent naphthalenyl-dimethoxybenzaldehyde enone compound that acts as a highly specific, sensitive, fast-responsive H₂S fluoroprobe (signal saturation at ≤ 5 min with a detection limit of 50 nM). Dose-dependently detects hydrogen sulfide levels under physiological conditions and readily fluoresces. Displays little to no reactivity towards GSH, Cys, H₂O₂, citrate and iodide (Hepes pH 7.4, containing 10% FBS). Also shown to be useful as a two-photon probe for imaging endogeneous H₂S levels in HeLa cells. A thiol specific fluorescent probe is also available (Cat. No. 595504).

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
hydrogen sulfide

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Regulatory Review (Z)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Singha, S., et al. 2015. Anal. Chem.87, 1188.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 405.44. Empirical Formula: C₂₄H₂₃NO₅. Assay: ≥. 98% (HPLC). Quality Level: 100. form: solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, protect from light. color: orange. solubility: DMSO: 100 . mg/mL. fluorescence: λ. _ex . 375 . nm, λ. _em . 505 . nm. storage temp.: 2-8°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.