Cell Line Origin
Rat hepatoma
Cell Line Description
H5 is a subclone of H4-II-E-C3 (Sigma Catalogue number. 85061112) which originated from the Reuber H-35 hepatoma induced by chemical carcinogenesis in an A x C rat. The dedifferentiated H5 cells express high levels of glucose-6-phosphate dehydrogenase and exhibit a population doubling time of 12 hours. Differentiated variants are H4IIEC3 (85061112), Fao and others.
Application
Toxicity studies; negative control for differentiated hepatoma
Culture Medium
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 1-2x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: rat liver. growth mode: Adherent. karyotype: Modal no. 52 (50-55). morphology: Fusiform. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94101905-1VL