Cell Line Origin
Mouse P3X63Ag8.653 x Mouse A.BY (Kb Ib Db) spleen
Cell Line Description
The antibody recognises a determinant on the H-2Dd molecule of B10.A(5R) mouse cells. The antibody also shows allopolymorphism linked to HLA-B7 and B27 in human cells.
Immunogen
A.TH (Ks Ik Dd) lymphoid cells
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder cells upon resuscitation
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: A.BY (Kb Ib Db) mouse spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-H-2Dd. technique(s): cell culture | mammalian: suitable. isotype: IgG2a, kappa. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51312503
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85062137-1VL