General description
Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Application
Hanks′ Balanced Salt Solution 10x has been used:
- as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium
- to wash the biopsy specimens of lamina propria lymphocytes (LPLs)
- as a wash solution to wash the fish cell line during removal from growth medium
Reconstitution
Supplement with 0.35 g/L sodium bicarbonate at 1X.
- UPC:
- 51132303
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- H4641-500ML