HCCBP1MAG-58K-05 MILLIPLEX(R) Human Circulating Cancer Biom

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology. MILLIPLEX(R) Circulating Cancer Biomarker Panel 1 is a 25-plex kit that can be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue/cell lysate and culture supernatant samples: AFP, CA125, CA15-3, CA19-9, CEA, CYFRA21-1, sFas, sFasL, FGF2, beta-HCG, HE4, HGF, IL-6, IL-8, Leptin, MIF, Osteopontin, Prolactin, PSA (free), PSA (total), SCF, TGFalpha, TNFalpha, TRAIL and VEGF. Note that certain biomarkers are tumor specific, such as PSA, while others, such as IL-8, have been detected in many cancers. PSA (free) and PSA (total) cannot be plexed together. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex(R) xMAP(R) platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Circulating Cancer Application: •Analytes: alpha-Fetoprotein (AFP), CA125, CA15-3, CA19-9, CEA, CYFRA21-1, sFas (TNFRSF6), sFasL, FGF-2 (FGF-basic), beta-HCG (HCGbeta), HE4, HGF, IL-6, IL-8 (CXCL8), Leptin, MIF, Osteopontin (OPN), Prolactin, PSA (free)*, PSA (total)*, SCF, TGFalpha, TNFalpha, TRAIL (TNFSF10), VEGF. •Note: Total PSA and Free PSA cannot be plexed together •Recommended Sample Type: Human serum, plasma or tissue/cell culture supernatants or lysates •Recommended Sample Dilution: 25 muL per well of 1:6 diluted plasma or serum, or 25 muL cell culture psaesaa sample undiluted or diluted in appropriate control medium •Assay Run Time: Overnight (16-18 hr) at 2-8C •Research Category: Cancer Legal Information: xMAP is a registered trademark of Luminex Corp Other Notes: Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.