Cell Line Origin
Human ileocecal adenocarcinoma
Cell Line Description
From a 67 year old male. The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.
Application
Tumourigenicity studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human intestine. growth mode: Adherent. karyotype: 2n : 46, pseudodiploid. morphology: Epithelial. products: Carcinoembryonic antigen (CEA). receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51172442
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90032006-1VL
