Heart Infusion Agar, 500G (C08-0444-011)

It is used for the isolation and cultivation of a wide variety of fastidious organisms. HI agar can also be used for the cultivation of Vibrio species. It can also be supplemented with glucose, horse serum and antibiotics for the cultivation a wide variety of organisms. It is used for mass cultivation of organisms for preparation of vaccines. On supplementation of blood, HI agar can be used to study haemolytic reactions. This medium was used for isolation and enumeration of haemolytic Streptococci in milk. Appearance: Cream to yellow homogeneous free flowing powder Gelling: Firm, comparable with 1.5% Agar gel Color and Clarity of prepared medium Basal medium : Light yellow colored, clear to slightly opalescent gel After addition of 5-7%w/v sterile defibrinated blood : Cherry red colored, opaque gel forms in Petri plates Reaction: Reaction of 4.0% w/v aqueous solution at 25°C. pH : 7.4±0.2 Cultural Response: Cultural characteristics observed with added 5-7% w/v sterile defibrinated blood, after an incubation at 35-37°C for 18-48 hours