Heart Infusion Agar, HiVeg, 500G

It is used for the isolation and cultivation of a wide variety of fastidious organisms. HI agar can also be used for the cultivation of Vibrio species. It can also be supplemented with glucose, horse serum and antibiotics for the cultivation a wide variety of organisms. It is used for mass cultivation of organisms for preparation of vaccines. On supplementation of blood, HI agar can be used to study haemolytic reactions. This medium was used for isolation and enumeration of haemolytic Streptococci in milk. Appearance of Powder: Light yellow colored, may have slightly greenish tinge, homogeneous, free flowing powder Gelling: Firm, comparable with 1.5% Agar gel of MV169 Color and Clarity: Basal medium yields light yellow color. With the addition of blood, cherry red colored opaque gel forms in petri plates, cherry red colored opaque solution in tubes Reaction: Reaction of 4.0% w/v of MV169 or 2.5% w/v of MV170 aqueous solution is pH 7.4 ± 0.2 at 25°C Cultural Response: Cultural characteristics observed after incubation at 35-37°C for 18-24 hours