Heart Infusion Broth, 500G (C08-0444-012)

The medium containing infusion from beef heart is used for the isolation and cultivation of a wide variety of fastidious organisms. It can also be used for the cultivation of Vibrio species. The medium can also be supplemented with glucose, horse serum and antibiotics for the cultivation a wide variety of organisms and to study carbohydrate fermentation. This medium was used for isolation and enumeration of haemolytic Streptococci in milk. Appearance: Cream to yellow homogeneous free flowing powder Color and Clarity of prepared medium: Basal medium: Light yellow colored, clear to slightly opalescent solution. After addition of 5% sterile defibrinated blood: Cherry red colored, opaque solution in tubes Reaction: Reaction of 2.5% w/v aqueous solution at 25°C. pH : 7.4±0.2 Cultural Response: Cultural characteristics observed with added 5%w/v sterile defibrinated blood, after an incubation at 35-37°C for 18-48 hours