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HEAT-LABILE COD URACIL-DNA GLYCOSYLASE (C005B-307005)

Catalog No.
C005B-307005
Mfr. No.
SRE0111-1000U
Mfr. Name
Sigma-Aldrich
Qty/UOM
1000
UOM
UN
Price: $1,618.50
List Price: $1,798.34

General description Uracil-DNA glycosylases are DNA repair enzymes that participate in the base excision repair pathway. Uracil DNA Glycosylase, also known as UNGor UDG, is an enzyme that hydrolyses the N-glycosylic bond between the deoxyribose

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General description

Uracil-DNA glycosylases are DNA repair enzymes that participate in the base excision repair pathway. Uracil DNA Glycosylase, also known as UNGor UDG, is an enzyme that hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic(apyrimidinic) site in DNA. Contamination of PCR samples with amplicon DNA generated by previous reactions, “carry-over contamination”, is one of the major sources of false positive results. Heat-labile Cod Uracil-DNA Glycosylase prevents carry-over contamination in PCR, qPCR, RT-qPCR, and RT-LAMP assays. The enzyme is produced in a recombinant E. coli (ung–) strain that contains a modified Cod UNGgene.

Application

Heat-labile Cod Uracil-DNA Glycosylase eliminates carry-over contamination in PCR, RT-PCR, RT-qPCR, qPCR and RT-LAMP

Features and Benefits

  • Heat inactivation: The only UNG that is completely and irreversibly heat inactivated thus ensuring long-term sample integrity for subsequent post-PCR analysis such as cloning, sequencing or genotyping
  • Cod UNG is not active on uracil in RNA.
  • High sensitivity: Remove more than 108 copies of contaminating DNA without affecting the sensitivity (Cq) of the assay
  • Simple protocol: Applying Cod UNG in a RT-PCR is done by a 5 min Cod UNG preincubation step at RT prior to the amplification reaction

Unit Definition

One Unit is defined as the amount of enzyme that liberates 1 nmol Uracil per hour from Uracil-labelled DNA at 37 °C in 70 mm Tris-HCI pH 8.0 (@25 °C), 10 mM NaCI, 1 mM EDTA and 0.1 mg/ml BSA.

Reconstitution

In solution

Other Notes

This product is for R&D use only. Not for drug, household, or other uses.

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UPC:
12352204
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
SRE0111-1000U
Temperature Control Device:
Yes

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