Cell Line Origin
Hepa-1c1c7, genetic modification, Xenobiotic-Responsive Element (XRE)
Cell Line Description
HeB5 is a derivative of Hepa-1c1c7 (Sigma product number 95090613) cell line stably transfected with a firefly luciferase reporter construct bearing Xenobiotic-Responsive Element (XRE) driven by a TATA promoter. HeB5 can be used for detection of mouse CYP1A induction by chemicals.
Culture Medium
Alpha-MEM + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16μg/ml Blasticidin S HCL.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
- UPC:
- 42203905
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 11033104-1VL