HEPES buffered saline (C15-1304-700)

General description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Application

HEPES buffered saline has been used:

• for the preparation of plasmid DNA
• as a reagent to prepare transfection master mix for DNA construct vectors
• to develop hydrogel precursor for cell leakage assay

Components

concentration (in H₂O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na₂HPO₄.2H₂O; 2.0 g/l dextrose

grade: for molecular biology. Quality Level: 200. product line: BioUltra. form: liquid. quality: 2× concentrate. composition: dextrose, 2.0 . g/L , HEPES, 10 . g/L , KCl, 0.74 . g/L , NaCl, 16 . g/L , Na₂HPO₄.2H₂O, 0.27 . g/L . concentration: 2 . ×. impurities: DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected. refractive index: n20/D 1.339. pH: 7.2 (25 . °C). cation traces: Al: ≤. 1 . mg/kg, Ba: ≤. 1 . mg/kg, Bi: ≤. 1 . mg/kg, Ca: ≤. 5 . mg/kg, Cd: ≤. 1 . mg/kg, Co: ≤. 1 . mg/kg, Cr: ≤. 1 . mg/kg, Cu: ≤. 1 . mg/kg, Fe: ≤. 1 . mg/kg, Li: ≤. 1 . mg/kg, Mg: ≤. 1 . mg/kg, Mn: ≤. 1 . mg/kg, Mo: ≤. 1 . mg/kg, Ni: ≤. 1 . mg/kg, Pb: ≤. 1 . mg/kg, Sr: ≤. 1 . mg/kg, Zn: ≤. 1 . mg/kg. λ: neat. UV absorption: λ. : 260 . nm A_max: 0.06, λ. : 280 . nm A_max: 0.04. SMILES string: OCCN1CCN(CC1)CCS(O)(: O): O. InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14). InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves.