General description
HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer."
In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO3). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.
Application
HEPES sodium salt has been used:
- to supplement the HSOF medium
- as a buffer component of HCZB medium in egg and embryo culture
- as a buffer component in cell culture
Features and Benefits
- High-purity product for biochemical and biological research
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
- Suitable for Cell culture
- Tested for Endotoxins and Total Aerobic Microbial Count
- Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease
Physical form
Lyophilized, spray dried, or crystallized material.
- UPC:
- 51342314
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- H3784-1KG
