General description
Research area: Cell Signaling
The hexokinase 1 (HXK1) gene is mapped to the Saccharomyces cerevisiae chromosome VI. HXK1 is ubiquitously expressed in mammalian tissues and is abundant in the brain, erythrocytes, lymphocytes, and fibroblasts.
Application
Hexokinase has been used:
- for digesting glucose for measuring glucose content in the root and stem samples of Quercus velutina
- to treat ADP (adenosine diphosphate) solution along with
D -glucose to remove the contaminating ATP (adenosine triphosphate) - in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.5) for ADP-[13CU]glucose synthesis
Biochem/physiol Actions
Catalyzes the phosphorylation of
The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.
Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.
Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
Hexokinase catalyzes the rate-limiting first step of glycolysis, which involves the phosphorylation of glucose.
Quality
A mixture of isoenzymes.
Unit Definition
One unit will phosphorylate 1.0 μmole of
Physical form
Lyophilized powder containing approx. 15% sodium citrate
Reconstitution
Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days.
- UPC:
- 41106622
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- H4502-500UN