Application

Product PLAK0645, Powered by Bethyl Laboratories, has been pre-qualified for use in proximity ligation assay (PLA^®). PLA^® technology enables visualization of protein interactions in fixed cells and tissue samples while under endogenous protein expression levels.

The secondary-conjugate (indirect) method of PLA^® requires the use of a pair of Duolink^® probes, one PLUS and one MINUS, and your choice of Detection Reagents. Accessories include Wash Buffers and Mounting Media.

Linkage

This kit contains antibody PLA0304 and antibody PLA0141, which have been pre-qualified as an antibody pair for use in Proximity Ligation Assay. To view all Duolink products, visit sigma-aldrich.com/duolink.

Analysis Note

The antibody for HIF1-alpha (PLA0304) is used at dilution 1:10,000 and the antibody for phospho-MCM2[S40/S41] (PLA0141) is used at dilution 1:250 for PLA applications.

Legal Information

Duolink is a registered trademark of Sigma-Aldrich Co. LLC

PLA is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.