High Affinity Ni-Charged Resin FF Prepacked Column,1ml x 2

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni 2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni 2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins. Storage Store in 20% ethanol at 2°C to 8°C. Do not freeze. Characteristics Binding capacity >40 mg of histidine-tagged protein /ml settled resin Matrix spherical 6% highly cross-linked agarose Average particle size 90 μm (45-165 μm) Storage solution 20% ethanol Storage and Stability 18 months when stored unopened at 2-8 °C   Column compatibility The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa). Reagents Compatible Denaturants Detergents Reducing agents Salts Others 6 M Gu·HCl 2% Triton X-100 20 mM β-ME 4 M MgCl₂ 50% glycerol 8 M Urea 2% Tween 20 1 mM DTT 5 mM CaCl₂ 20% ethanol   1% CHAPS   2 M NaCl 1 mM EDTA