DescriptionLipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.Suitability: Highly sensitive kit suitable for the measurement of lipolysis in 3T3-L1 and adipocytes.Principle: The High Sensitivity Lipolysis (3T3-L1) Fluorometric Assay Kit contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent product (λex= 535/ λem= 587 nm) proportional to the amount of glycerol present.}. Specification: Sufficient for 100 Fluorometric tests Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/H486406-SCI_f999771524785a3049a6792814518646.pdf
- UPC:
- 41141822
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 3.53 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- H486406-1kit
- Product Size:
- 1kit
- Hazard Statement Codes:
- H315:H319:H335
- Precautionary Statement Codes:
- P261:P305+P351+P338
