General description
His GraviTrap™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.
The ready to use column is prepacked with Ni Sepharose™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.
His GraviTrap™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.
Features and Benefits
- For fast and simple purification of histidine-tagged* proteins.
- Convenient, prepacked gravity-flow column.
- Allows direct purification of both clarified and unclarified cell lysates.
- High binding capacity; approximately 40 mg of pure protein per column.
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives.
Storage and Stability
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
- UPC:
- 51342803
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- GE11-0033-99
