General description
Capto™ Phenyl (High sub) and Capto™ Butyl are hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification.
Application
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration.
Features and Benefits
- Prepacked with Capto™ Butyl
- Excellent for method optimization and parameter screening due to the 10 cm bed height
- Easily connected in series to achieve 20 cm bed height
- Small bed volume for fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity
Storage and Stability
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
- UPC:
- 51442721
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- GE28-9924-73
