General description
Histopaque-1077 is a sterile, endotoxin tested, ready-to-use medium. The Histopaque composition includes polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.
Application
Histopaque-1077 is suitable for human lymphocyte antigen (HLA) typing and as the initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes. It may also be employed in the preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
Histopaque-1077 has been used for:
- the isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
- purification of mononuclear cells through centrifugation
- isolation of human lymphocytes
- isolation of breast cancer circulating tumor cells (CTCs) from the whole blood
Features and Benefits
Histopaque®-1077 offers numerous advantages, such as:
- Optimal recovery of viable cells
- Permits selective separation of blood cell types
- Prevents cell distortion
- Preserves cell viability
- Minimal extraneous cell interference
- Reproducible performance from batch to batch
- Sterile-filtered and endotoxin-tested
- Stable at 2-8°C for at least 2 years when protected from light
Principle
Anticoagulated blood is layered ontoHistopaque-1077. During centrifugation, erythrocytes are aggregated by polysucroseand rapidly sediment. Granulocytes becomeslightly hypertonic, which increases their sedimentation rate, resulting in pelleting atthe bottom of the centrifuge tube. Lymphocytes and other mononuclear cells remain at the plasma/ Histopaque-1077interface.
Legal Information
Histopaque is a registered trademark of Sigma-Aldrich Co. LLC
- UPC:
- 41115709
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10771-6X100ML
