General description
Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.
Application
Histopaque®-1077 Hybri-Max™ has been used to isolate:
- peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
- immune cells (lymphocytes) from peripheral blood
- PBMCs from blood
Legal Information
Histopaque is a registered trademark of Sigma-Aldrich Co. LLC
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
- UPC:
- 41113010
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- H8889-500ML