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histopaque(r)-1083 (c15-1296-297)

Histopaque(R)-1083 (C15-1296-297)

Catalog No.
C15-1296-297
Manufacturer No.
10831-6X100ML
Manufacturer Name
Sigma-Aldrich
Quantity
600
Unit of Measure
ML
Price: $519.81
List Price: $577.57

Histopaque ® -1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.083 g/mL.

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General description

Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Application

Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Features and Benefits

Histopaque®-1083 offers numerous advantages, such as:

  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Principle

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes areaggregated by polysucrose and rapidly sediment;whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

UPC:
41131722
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
10831-6X100ML


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