Histopaque(R)-1119 (C15-1296-907)

General description

Histopaque^®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a density of 1.119 g/mL and pH 8.8–9.0.

Application

Histopaque^®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood.
A double gradient is formed by layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto theupper Histopaque-1077 medium. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment. Granulocytes are found at the lowerHistopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upperplasma/Histopaque-1077 interface.It is also suitable for the purification of dendritic cells from chicken bone marrow cell suspension and neutrophils from mouse bone marrow cell suspension

Features and Benefits

Histopaque^®-1119 offers numerous advantages, such as:

• Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
• Sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light

Principle

The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible.

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC