General description
Contains amino acid residues [77-436] of HIV-1 gag antigen.
The human immunodeficiency virus 1 (HIV−1) gag antigen is a recombinant 39 kDa protein encoded by the GAG gene of the human immunodeficiency virus (HIV) type I expressed in and isolated from Escherichia coli. It has a β−galactosidase tag (114 kDa) on the N−terminus. HIV-1 Gag exists as monomers or lower-order oligomers in the cytosol. At the plasma membrane, it forms higher-order multimers.
Application
HIV-1 gag has been used in the coculture with an autologous cluster of differentiation T (CD4+ T) to study the ability of monocyte-derived dendritic cells (MDC1) to induce human immunodeficiency virus 1 (HIV−1) latency reversal (LR).
Biochem/physiol Actions
The human immunodeficiency virus 1 (HIV−1) gag (p24) antigen reacts strongly with HIV positive serum and may be used as a positive control for evaluating antibodies to HIV. At transcription sites, HIV-1 Gag creates ribonucleoprotein complexes with unspliced viral RNA. The HIV-1 Gag protein is accountable for packaging genomic RNA (gRNA) and the assembly of immature viral particles.
Positive control for HIV antibodies.
Physical form
Solution in 8 M urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.
- UPC:
- 42293104
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- H0160-100UG
