HiVeg Hydrolysate No. 4, 2.5kg

It is rich in amino acids and can be successfully used to supplement Tissue culture Media and therefore for production of vaccines of viral origin including foot and mouth disease, polio, dengue, Coxsackie B3 and many other viruses. It can be used as a substrate for many microorganisms including Lactobacilli, for sporulation of Clostridia and fermentation procedures. Principle And Interpretation HiVeg Hydrolysate No. 4 is prepared under controlled condition from vegetable proteins that can successfully replace Lactalbumin Hydrolysate (RM012). Quality Control Appearance LLight yellow to yellow, may have slight green tinge Homogenous Free flowing powder, having Characteristic odour. Solubility Freely soluble in distilled/ purified water, insoluble in alcohol, chloroform. Clarity 1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC ) for 15 minutes. Reaction Reaction of 2%w/v aqueous solution at 25ºC. pH 5.50- 7.50 Microbial Load: Total aerobic microbial count (cfu/gm) By plate method when incubated at 30-35°C for not less than 3 days. Bacterial Count : <= 2000 CFU/gram Total Yeast and mould count (cfu/gm) By plate method when incubated at 20-25°C for not less than 5 days. Yeast & mould Count : <= 100 CFU/gram Test for Pathogens 1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosa- Negative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of sample6. Clostridia- Negative in 10 gms of sample Indole test Tryptophan content: Passes Cultural response The growth promoting capacity of the medium containing HiVeg Hydrolysate No. 4 with Hank's Salts or Earle's Salts is assessed by supplementing it with 10% fetal bovine serum using an appropriate cell line and comparing it with control medium. Growth promotion studies are carried out through two or more subcultures.Cells are counted and growth curve is plotted as a logarithmic function of time in culture. The medium is also tested for its good anchoraging capacity and buffering action. Storage and Shelf Life Store below 30°C. Use before expiry date on the label.