HiVeg Peptone No. 1, 5kg

It is recommended for use in general purpose culture media for routine cultivation of a variety of microorganisms. It can also be used in mass scale cultivation of microorganisms for antibiotics, enzymes, vitamins production or for other similar products of microbial origin. Principle And Interpretation HiVeg Peptone No. 1 is an enzymic hydrolysate of specially selected vegetable proteins. It is recommended for use in bacteriological culture media that can successfully replace meat peptones (RM635). Quality Control Appearance Light yellow to yellow may have slight green tinge, Homogenous Free flowing powder having Characteristic odour but not putrescent. Solubility Freely soluble in distilled/ purified water, insoluble in alcohol. Clarity 1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC ) for 15 minutes. Reaction Reaction of 2%w/v aqueous solution at 25ºC. pH 5.50- 7.50 Microbial Load: Total aerobic microbial count (cfu/gm) By plate method when incubated at 30-35°C for not less than 3 days. Bacterial Count : <= 2000 CFU/gram Total Yeast and mould count (cfu/gm) By plate method when incubated at 20-25°C for not less than 5 days. Yeast & mould Count : <= 100 CFU/gram Test for Pathogens 1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosa- Negative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of sample6. Clostridia- Negative in 10 gms of sample Indole test Tryptophan content: Passes Cultural response Cultural response observed after incubation at 35 - 37°C for 18-48 hours by preparing Nutrient HiVeg Agar (MV001), using HiVeg Peptone as an ingredient. Storage and Shelf Life Store below 30°C. Use before expiry date on the label.