HiVeg Peptone No. 3, 5kg

It is highly nutritious and can be employed in culture media for bulk production of antibiotics, enzymes, veterinary preparations, bacterial toxins, etc. It is recommended for use in media that support good growth of a large number of microorganisms including Staphylococci, Streptococci, Pneumococci, Meningococci, Gonococci, among others which require a highly nutritious medium. Principle And Interpretation HiVeg Peptone No. 3 is an enzymic hydrolysate of vegetable proteins and recommended for cultivation of fastidious pathogens. It can successfully replace Proteose peptone (RM005). Quality Control Appearance Light yellow to yellow, may have a slight greenish tinge Homogenous Free flowing powder ,having Characteristic odour of protein, derived from vegetable source. Solubility Freely soluble in distilled/purified water,insoluble in alcohol and ether. Clarity 1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC ) for 15 minutes. Reaction Reaction of 2%w/v aqueous solution at 25ºC. pH 5.50- 7.50 Microbial Load: Total aerobic microbial count (cfu/gm) By plate method when incubated at 30-35°C for not less than 3 days. Bacterial Count : <= 2000 CFU/gram Total Yeast and mould count (cfu/gm) By plate method when incubated at 20-25°C for not less than 5 days. Yeast & mould Count : <= 100 CFU/gram Test for Pathogens 1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosa- Negative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of sample6. Clostridia- Negative in 10 gms of sample Indole test Tryptophan content: Passes Cultural response Cultural response observed after incubation at 35 - 37°C for 18-48 hours by preparing Nutrient HiVeg Agar (MV001), using HiVeg Peptone as an ingredient. Storage and Shelf Life Store below 30°C. Use before expiry date on the label.