HiVeg Peptone No. 4, 2.5kg

Recommended for use as culture media ingredient in variety of media for cultivation of yeasts and moulds. Principle And Interpretation HiVeg Peptone No. 4 is prepared under controlled condition from vegetable proteins. It is highly nutritious and supports heavy growth of a wide variety of microorganisms comparable with Peptone M (RM006). Quality Control Appearance Light yellow to yellow, may have a slight green tinge Homogenous Free flowing powder, having Characteristic odour of protein, derived from vegetable source. Solubility Freely soluble in distilled/purified water,insoluble in alcohol and ether. Clarity 1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC ) for 15 minutes. Reaction Reaction of 2%w/v aqueous solution at 25ºC. pH 5.50- 7.50 Microbial Load: Total aerobic microbial count (cfu/gm) By plate method when incubated at 30-35°C for not less than 3 days. Bacterial Count : <= 2000 CFU/gram Total Yeast and mould count (cfu/gm) By plate method when incubated at 20-25°C for not less than 5 days. Yeast & mould Count : <= 100 CFU/gram Test for Pathogens 1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosa- Negative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of sample6. Clostridia- Negative in 10 gms of sample Indole test Tryptophan content: Passes Cultural response Cultural response observed after incubation at 25-30°C for 48-72 hours by preparing Malt Extract HiVeg Agar (MV137) using HiVeg Peptone No.4 as an ingredient. Storage and Shelf Life Store below 30°C. Use before expiry date on the label.