General description
Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.
Application
Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.
Components
EC 2.7.1.1 & 1.1.1.49
Unit Definition
One unit (U) HK will phosphorylate 1 μmol of D-glucose in one minute at +25 °C and pH 7.6.
One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated.
Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.
Other Notes
For life science research only. Not for use in diagnostic prodcedures.
- UPC:
- 41115701
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10737275001
