Cell Line Origin
Human Caucasian promyelocytic leukaemia
Cell Line Description
Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.
Application
Differentiation studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Do not allow the culture medium to become acidic.
Other Notes
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biological source: human blood. growth mode: Suspension. karyotype: 2n : 48, hyperdiploid. morphology: Promyeloid, blast/promyelocyte. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51131631
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 88120802-1VL
