HMS174(DE3)PLYSS COMPETENT CELLS - NOVAGEN (C15-1307-773)

General description

Genotype: F^-recA1 hsdR(r_K12^- m_K12⁺) (DE3) pLysS (Cam^R, Rif ^R)





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HMS174 strains provide the recA mutation in aK-12 background. Like BLR, these strains maystabilize certain target genes whose productsmay cause the loss of the DE3 prophage.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlHMS174(DE3)pLysS Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: Escherichia coli. manufacturer/tradename: Novagen^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. growth mode: adherent or suspension. morphology: rod shaped. technique(s): microbiological culture: suitable. cell transformation: transformation efficiency: >. 5×10⁶ . cfu/μ. g. shipped in: dry ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.