HPLC column (analytical), NUCLEOSIL 100-10 CN, length: 250 m (C08-0494-607)

NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure Wide acceptance as routine packings for very different fields of chromatography One of the first spherical silicas used in HPLC Developed in the early seventies, it became a world-renowned HPLC packing Still found in many analytical and preparative application An absolutely reliable choice in HPLC The largest variety of modified HPLC silicas available on the market NUCLEOSIL® CN / CN-RP Cyano (nitrile) modified silica phases for HPLC USP L10 Features Polar to mid-polar cyano (nitrile) modified silica for reversed phase and normal phase chromatography Normal phase: with low-polarity solvents for many compounds, which can also be separated on unmodified silica, however, due to the rapid equilibration much more suitable for gradient separations Reversed phase: with different selectivity than C18, C8 or phenyl modified packings Available pore sizes 100 and 120 Å Available particle sizes 5 µm, 7 µm and 10 µm pH stability at 20 °C: 2–8 Carbon content 5 % (100 Å), ~ 3 % (120 Å) Eluent in column CN: n-heptane CN-RP: acetonitrile – water Separation mechanism based on π–π interactions, polar interactions and hydrophobic interactions Corresponding NUCLEODUR® phase see NUCLEODUR® CN / CN-RP